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1. A method is described for obtaining pure cultures of Treponema pallidum and allied species of spirochætæ in fluid media.
2. The principle of the method is based upon the superposition of a favorable culture medium upon a less favorable one.
3. The method here described is not suited to the growth of spirochætæ when they are admixed with contaminating bacteria.
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Copyright, 1912, by The Rockefeller Institute for Medical Research New York
1912
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