Multiple cell wall blebs were observed on the surface of three strains of N. meningitidis taken from log phase cultures. The blebs originated as evaginations of the outer layer of the cell wall. Bleb production was noted on both defined or complex media either as broth or a solid medium. The addition of 10% normal bovine serum to the various media did not affect the production and release of these surface blebs. However, as broth cultures progressed into the stationary phase of growth, the blebs disappeared from the surface of the cells. Blebs were present in substantial quantities in culture supernatant fluids and on cell surfaces and were readily isolated by ultracentrifugation. Analysis for 2-keto-3-deoxyoctonate in cultures revealed that 18% of the total endotoxin of log phase cultures was present in blebs from the cell wall.
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1 November 1973
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November 01 1973
RELEASE OF ENDOTOXIN IN THE FORM OF CELL WALL BLEBS DURING IN VITRO GROWTH OF NEISSERIA MENINGITIDIS
I. W. Devoe,
I. W. Devoe
From the Department of Microbiology, Macdonald Campus of McGill University, Macdonald College 800, Quebec, Canada
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J. E. Gilchrist
J. E. Gilchrist
From the Department of Microbiology, Macdonald Campus of McGill University, Macdonald College 800, Quebec, Canada
Search for other works by this author on:
I. W. Devoe
From the Department of Microbiology, Macdonald Campus of McGill University, Macdonald College 800, Quebec, Canada
J. E. Gilchrist
From the Department of Microbiology, Macdonald Campus of McGill University, Macdonald College 800, Quebec, Canada
Received:
July 25 1973
Online ISSN: 1540-9538
Print ISSN: 0022-1007
Copyright © 1973 by The Rockefeller University Press
1973
J Exp Med (1973) 138 (5): 1156–1167.
Article history
Received:
July 25 1973
Citation
I. W. Devoe, J. E. Gilchrist; RELEASE OF ENDOTOXIN IN THE FORM OF CELL WALL BLEBS DURING IN VITRO GROWTH OF NEISSERIA MENINGITIDIS . J Exp Med 1 November 1973; 138 (5): 1156–1167. doi: https://doi.org/10.1084/jem.138.5.1156
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