IMMUNOCHEMICAL STUDIES ON BLOOD GROUPS LI. A COMPARATIVE STUDY OF THE REACTION OF A1 AND A2 BLOOD GROUP GLYCOPROTEINS WITH HUMAN ANTI-A

Moreno , Carlos; Lundblad , Arne; Kabat , Elvin A.

doi:10.1084/jem.134.2.439

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Article| August 01 1971

IMMUNOCHEMICAL STUDIES ON BLOOD GROUPS : LI. A COMPARATIVE STUDY OF THE REACTION OF A₁AND A₂ BLOOD GROUP GLYCOPROTEINS WITH HUMAN ANTI-A

Carlos Moreno,

Carlos Moreno

From the Departments of Microbiology, Neurology, and Human Genetics and Development, College of Physicians and Surgeons, Columbia University, and the Neurological Institute, Presbyterian Hospital, New York 10032

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Arne Lundblad,

Arne Lundblad

From the Departments of Microbiology, Neurology, and Human Genetics and Development, College of Physicians and Surgeons, Columbia University, and the Neurological Institute, Presbyterian Hospital, New York 10032

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Elvin A. Kabat

From the Departments of Microbiology, Neurology, and Human Genetics and Development, College of Physicians and Surgeons, Columbia University, and the Neurological Institute, Presbyterian Hospital, New York 10032

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Author and Article Information

Carlos Moreno

From the Departments of Microbiology, Neurology, and Human Genetics and Development, College of Physicians and Surgeons, Columbia University, and the Neurological Institute, Presbyterian Hospital, New York 10032

Arne Lundblad

From the Departments of Microbiology, Neurology, and Human Genetics and Development, College of Physicians and Surgeons, Columbia University, and the Neurological Institute, Presbyterian Hospital, New York 10032

Elvin A. Kabat

From the Departments of Microbiology, Neurology, and Human Genetics and Development, College of Physicians and Surgeons, Columbia University, and the Neurological Institute, Presbyterian Hospital, New York 10032

Received: May 03 1971

Online ISSN: 1540-9538

Print ISSN: 0022-1007

1971

J Exp Med (1971) 134 (2): 439–457.

https://doi.org/10.1084/jem.134.2.439

The immunochemical properties of purified A₁ and A₂ glycoproteins have been compared to ascertain whether their antigenic determinants differ. Quantitative precipitin and complement-fixation studies using several anti-A sera as well as purified γG anti-A antibodies clearly showed a specificity difference. This was also supported by absorption studies: A₂ substance specifically removed antibodies reacting with A₂ substance leaving anti-A₁ activity. A₁ substance was more effective than A₂ substance in dissolving an A₁ anti-A₁-specific precipitate. Purified γM anti-A hemolyzed A₁ cells more readily than A₂ cells. Inhibition studies using mono- and difucosyl type 2 A-active oligosaccharides showed that type 2 difucosyl receptors are present in A₂ substance. The structural basis for the specificity difference between A₁ and A₂ would appear to be that A₂ substances lack type 1 A determinants; this would account for the observed higher H and Le^b activity in A₂ substances.

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IMMUNOCHEMICAL STUDIES ON BLOOD GROUPS : LI. A COMPARATIVE STUDY OF THE REACTION OF A1 AND A2 BLOOD GROUP GLYCOPROTEINS WITH HUMAN ANTI-A

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IMMUNOCHEMICAL STUDIES ON BLOOD GROUPS : LI. A COMPARATIVE STUDY OF THE REACTION OF A₁AND A₂ BLOOD GROUP GLYCOPROTEINS WITH HUMAN ANTI-A