We have investigated the in vitro transformation of lymphocytes that have been frozen and stored at low temperatures. Cells frozen at different times and stored for various times do transform in a reproducible manner when placed in culture with PHA or as one population of the two-way or the one-way MLR.
When frozen-stored lymphocytes are cultured with specific antigen or as both partners in the MLR the response is minimal.
The freezing process destroys neutrophils, and the remaining population transforms in a manner similar to cultures of purified lymphocytes.
Practical aspects of the technique are discussed and we have suggested possible practical applications for future investigation.
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Copyright © 1970 by The Rockefeller University Press
1970
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