The antigen reacting with complement-fixing antibodies in the sera of patients with primary biliary cirrhosis was localized predominantly in the mitochondrial fraction of tissue homogenates obtained by differential centrifugation. Purified mitochondrial preparations had a high content of the antigen whereas purified lysosomes failed to fix complement with PBC sera. Analysis of a number of fractionation experiments showed a high correlation between antigen content and the mitochondrial enzyme succinic dehydrogenase in all fractions. There was much poorer correlation with lysosomal and micrososomal enzyme markers. The patterns of staining obtained with a fluorescein conjugate of IgG from a PBC patient closely paralleled those obtained with a histochemical method for the demonstration of succinic dehydrogenase, further confirming the mitochondrial localization of the antigen. Staining was brightest in cells containing mitochondria with well-developed cristae. Studies on mitochondria fragmented by osmotic lysis, hexane, lysolecithin, and ultrasound suggest that the antigen is associated with the mitochondrial inner membranes.
Skip Nav Destination
Article navigation
1 August 1967
Article|
August 01 1967
MITOCHONDRIAL ANTIBODIES IN PRIMARY BILIARY CIRRHOSIS : I. LOCALIZATION OF THE ANTIGEN TO MITOCHONDRIAL MEMBRANES
Peter A. Berg,
Peter A. Berg
From the Rheumatology Research Department, Middlesex Hospital Medical School, London, England
Search for other works by this author on:
Deborah Doniach,
Deborah Doniach
From the Rheumatology Research Department, Middlesex Hospital Medical School, London, England
Search for other works by this author on:
Ivan M. Roitt
Ivan M. Roitt
From the Rheumatology Research Department, Middlesex Hospital Medical School, London, England
Search for other works by this author on:
Peter A. Berg
From the Rheumatology Research Department, Middlesex Hospital Medical School, London, England
Deborah Doniach
From the Rheumatology Research Department, Middlesex Hospital Medical School, London, England
Ivan M. Roitt
From the Rheumatology Research Department, Middlesex Hospital Medical School, London, England
Received:
March 30 1967
Online ISSN: 1540-9538
Print ISSN: 0022-1007
Copyright © 1967 by The Rockefeller University Press
1967
J Exp Med (1967) 126 (2): 277–290.
Article history
Received:
March 30 1967
Citation
Peter A. Berg, Deborah Doniach, Ivan M. Roitt; MITOCHONDRIAL ANTIBODIES IN PRIMARY BILIARY CIRRHOSIS : I. LOCALIZATION OF THE ANTIGEN TO MITOCHONDRIAL MEMBRANES . J Exp Med 1 August 1967; 126 (2): 277–290. doi: https://doi.org/10.1084/jem.126.2.277
Download citation file:
Sign in
Don't already have an account? Register
Client Account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Sign in via your Institution
Sign in via your InstitutionSuggested Content
Email alerts
Advertisement