Peritoneal cells obtained from animals exhibiting delayed hypersensitivity are inhibited from migrating in vitro by specific sensitizing antigen. This test for the detection of delayed hypersensitivity was applied to the problem of cellular sensitivity in allergic encephalomyelitis (AE). The migration of peritoneal cells obtained from guinea pigs with AE was inhibited specifically by nervous tissue antigens. The specificity of this reaction was further studied. Neonatal rat nervous tissue, which was shown to lack the encephalitogenic antigen, i.e. did not produce AE when injected with complete adjuvant into guinea pigs and rats, did not inhibit the migration of cells from animals with AE. Adult rat nervous tissue, which readily produces AE, and thus contains the encephalitogenic antigen did inhibit the migration of such cells. The finding that cells from animals with AE display hypersensitivity which appears to be directed specifically to the encephalitogenic antigen strongly supports the view that such cells could play an important role in the pathogenesis of this disease.
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1 December 1965
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December 01 1965
IN VITRO DEMONSTRATION OF CELLULAR SENSITIVITY IN ALLERGIC ENCEPHALOMYELITIS
John R. David,
John R. David
From the Department of Medicine, New York University School of Medicine, New York
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Philip Y. Paterson
Philip Y. Paterson
From the Department of Medicine, New York University School of Medicine, New York
Search for other works by this author on:
John R. David
From the Department of Medicine, New York University School of Medicine, New York
Philip Y. Paterson
From the Department of Medicine, New York University School of Medicine, New York
Received:
August 17 1965
Online ISSN: 1540-9538
Print ISSN: 0022-1007
Copyright © 1965 by The Rockefeller University Press
1965
J Exp Med (1965) 122 (6): 1161–1171.
Article history
Received:
August 17 1965
Citation
John R. David, Philip Y. Paterson; IN VITRO DEMONSTRATION OF CELLULAR SENSITIVITY IN ALLERGIC ENCEPHALOMYELITIS . J Exp Med 1 December 1965; 122 (6): 1161–1171. doi: https://doi.org/10.1084/jem.122.6.1161
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