COLICINE K : III. THE IMMUNOLOGICAL PROPERTIES OF A SUBSTANCE HAVING COLICINE K ACTIVITY

By immunological means it has been shown that colicine K is associated with the O antigen of the colicinogenic bacillus E. coli K₂₃₅ L₊OC₊.

The colicine K-O antigen complex elicits the formation of at least two types of antibodies, one a precipitin, the other a colicine-neutralizing antibody. The first precipitates colicine K without neutralizing it, the second neutralizes the colicine without precipitating it.

Unlike the purified colicine K complex, the colicine protein component of the O antigen is precipitable by the neutralizing antibody.

There is no demonstrable serological relationship between colicine K and phage T₆. These two agents must be considered to be separate and distinct entities.