The inactivation of purified plasmin by serum or plasma was studied using a caseinolytic assay for determination of plasmin. On the basis of kinetic evidence, the presence of two inhibitors in normal human or guinea pig serum or plasma is suggested. The first reacts immediately at any temperature, but is easily dissociable and does not combine with plasmin in a fixed ratio. The second combines more slowly with plasmin at a rate which depends on temperature and on the concentration of both plasmin and inhibitor. There is a fixed ratio of reaction between plasmin and the second inhibitor and the combination does not readily dissociate. There is sufficient of the "slow" inhibitor in normal plasma to inactivate more than 30 times its own content of potential plasmin. The presence of suitable substrate for plasmin tends to reverse the combination with "immediate" inhibitor and to stop the further action of "slow" inhibitor.
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1 July 1958
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July 01 1958
STUDIES OF THE PLASMIN SYSTEM : II. INHIBITION OF PLASMIN BY SERUM OR PLASMA
Philip S. Norman
Philip S. Norman
From the Biological Division, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, and The Rockefeller Institute for Medical Research
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Philip S. Norman
From the Biological Division, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, and The Rockefeller Institute for Medical Research
Received:
March 10 1958
Online ISSN: 1540-9538
Print ISSN: 0022-1007
Copyright, 1958, by The Rockefeller Institute for Medical Research New York
1958
J Exp Med (1958) 108 (1): 53–68.
Article history
Received:
March 10 1958
Citation
Philip S. Norman; STUDIES OF THE PLASMIN SYSTEM : II. INHIBITION OF PLASMIN BY SERUM OR PLASMA . J Exp Med 1 July 1958; 108 (1): 53–68. doi: https://doi.org/10.1084/jem.108.1.53
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