A STREPTOCOCCAL ENZYME THAT ACTS SPECIFICALLY UPON DIPHOSPHOPYRIDINE NUCLEOTIDE: CHARACTERIZATION OF THE ENZYME AND ITS SEPARATION FROM STREPTOLYSIN O

Carlson , Arthur S.; Kellner , Aaron; Bernheimer , Alan W.; Freeman , Elizabeth B.

doi:10.1084/jem.106.1.15

Article navigation

Article| July 01 1957

A STREPTOCOCCAL ENZYME THAT ACTS SPECIFICALLY UPON DIPHOSPHOPYRIDINE NUCLEOTIDE: CHARACTERIZATION OF THE ENZYME AND ITS SEPARATION FROM STREPTOLYSIN O

Arthur S. Carlson,

Arthur S. Carlson

From the Department of Pathology, The New York Hospital-Cornell Medical Center, and The Department of Microbiology, New York University College of Medicine, New York

Search for other works by this author on:

This Site

PubMed

Google Scholar

Aaron Kellner,

Aaron Kellner

From the Department of Pathology, The New York Hospital-Cornell Medical Center, and The Department of Microbiology, New York University College of Medicine, New York

Search for other works by this author on:

This Site

PubMed

Google Scholar

Alan W. Bernheimer,

Alan W. Bernheimer

From the Department of Pathology, The New York Hospital-Cornell Medical Center, and The Department of Microbiology, New York University College of Medicine, New York

Search for other works by this author on:

This Site

PubMed

Google Scholar

Elizabeth B. Freeman

From the Department of Pathology, The New York Hospital-Cornell Medical Center, and The Department of Microbiology, New York University College of Medicine, New York

Search for other works by this author on:

This Site

PubMed

Google Scholar

Author and Article Information

Arthur S. Carlson

From the Department of Pathology, The New York Hospital-Cornell Medical Center, and The Department of Microbiology, New York University College of Medicine, New York

Aaron Kellner

From the Department of Pathology, The New York Hospital-Cornell Medical Center, and The Department of Microbiology, New York University College of Medicine, New York

Alan W. Bernheimer

From the Department of Pathology, The New York Hospital-Cornell Medical Center, and The Department of Microbiology, New York University College of Medicine, New York

Elizabeth B. Freeman

From the Department of Pathology, The New York Hospital-Cornell Medical Center, and The Department of Microbiology, New York University College of Medicine, New York

Received: February 20 1957

Online ISSN: 1540-9538

Print ISSN: 0022-1007

Copyright, 1957, by The Rockefeller Institute for Medical Research New York

1957

J Exp Med (1957) 106 (1): 15–26.

https://doi.org/10.1084/jem.106.1.15

All enzyme that destroys DPN has been found in fractionated and highly concentrated streptococcal preparations that also contain streptolysin O. The enzyme—streptococcal DPNase—was shown by electrophoretic separation and by other means to be distinct from streptolysin O. It is non-dialyzable, heat-labile, and has optimal activity in the pH range 7.2 to 7.8. The enzyme has a high degree of specificity for DPN, which it splits at the nicotinamide-ribose linkage.

This content is only available as a PDF.

Copyright, 1957, by The Rockefeller Institute for Medical Research New York

1957

You do not currently have access to this content.

Don't already have an account? Register

You could not be signed in. Please check your email address / username and password and try again.

A STREPTOCOCCAL ENZYME THAT ACTS SPECIFICALLY UPON DIPHOSPHOPYRIDINE NUCLEOTIDE: CHARACTERIZATION OF THE ENZYME AND ITS SEPARATION FROM STREPTOLYSIN O

Sign in

Client Account

Sign in via your Institution

Suggested Content

Email alerts

This Feature Is Available To Subscribers Only