Figure 1. Genetically engineered mouse model lacking the first four domains of titin’s P-zone, A164–167. (A) Titin’s A-band is incorporated into the thick filament, with the P-zone linking the C-zone and M-band segments, spanning domains More about this image found in Genetically engineered mouse model lacking the first four domains of titin’...

Figure 2. RNA sequencing and fiber typing results. (A) GO terms associated with the DEGs in LV tissue for CC (pink), BP (orange), and MF (peach) categories. Significantly enriched GO terms were determined by Fisher’s exact test. 4 WT and 4 Ttn More about this image found in RNA sequencing and fiber typing results. (A) GO terms associated with the ...

Figure 3. Single EDL fiber mechanics. (A) Peak passive stress produced by single skinned EDL fibers across a range of sarcomere lengths. (B) Elastic component of passive stress. (C) Viscous component of passive stress. Statistical More about this image found in Single EDL fiber mechanics. (A) Peak passive stress produced by single ski...

Figure 4. Cardiomyocyte mechanics. (A) Peak stress generated by cardiomyocytes passively stretched to a range of sarcomere lengths. (B) Elastic component of passive stress. (C) Viscous component of passive stress. Statistical significance More about this image found in Cardiomyocyte mechanics. (A) Peak stress generated by cardiomyocytes passi...

Figure 5. Intact EDL studies. (A) Force–frequency relationship of intact EDL muscle with PCSA measurement inset. The force–frequency curves were fit with a Hill equation, and statistical significance for curve fit was determined by an extra More about this image found in Intact EDL studies. (A) Force–frequency relationship of intact EDL muscle ...

Figure 6. Cardiac function and morphology. (A) Fractional shortening, isovolumic relaxation time normalized to RR time interval (IVRT/RR interval), myocardial performance index (MPI), mitral valve E-wave deceleration time (MV E decel. time), More about this image found in Cardiac function and morphology. (A) Fractional shortening, isovolumic rel...

Figure 7. Mapping titin’s A-band epitopes in Ttn ^ΔA164–167 cardiac muscle. (A) Schematic of titin’s A-band (M-band on left) with summary of epitopes used to map titin’s arrangement in the A-band. (B) Representative immunoelectron micrograph More about this image found in Mapping titin’s A-band epitopes in Ttn ^ΔA164...

Figure 8. Measuring thick filament length and mapping cMyBP-C. (A) Representative electron micrographs of WT (left) and Ttn^ΔA164–167 (right) sarcomeres, with plot profiles below in which the length of the A-band was measured as indicated by the More about this image found in Measuring thick filament length and mapping cMyBP-C. (A) Representative el...

Figure 9. Proposed model of the WT (A) and Ttn^ΔA164–167 (B) thick filament. (A and B) The thick filament components (titin, myosin, and cMyBP-C) are shown vertically stacked for clarity (labels to the right). The myosin molecules in the P-zone More about this image found in Proposed model of the WT (A) and Ttn^ΔA164–167...

Figure 1. Cholesterol regulates membrane excitability and mechanical hypersensitivity in naïve and neuropathic pain rats. (A–C) Representative action potential firing of non-spontaneously (A) and spontaneously (B) firing naïve DRG neurons before More about this image found in Cholesterol regulates membrane excitability and mechanical hypersensitivity...

Figure 2. Combining the CTxB-based FRET approach and a cholesterol sensor in assessing the modulation of PM properties by cholesterol enrichment. (A) Cartoon showing the FRET between CTxB AF-488 and CTxB AF-647 under conditions of small OMDs and More about this image found in Combining the CTxB-based FRET approach and a cholesterol sensor in assessin...

Figure 3. Distinct modes of PM modulation by cholesterol enrichment. (A) Strategies implemented to report the PM properties after WSC-mediated cholesterol supplementation. An mCherry-labeled OlyA was used to recognize outer leaflet More about this image found in Distinct modes of PM modulation by cholesterol enrichment. (A) Strategies ...

Figure 4. Fluorescence anisotropy reports homo-FRET between cholesterol sensors. (A) Representative confocal images showing membrane-localized eGFP-GRAW–W fluorescence emission in a naïve DRG neuron, acquired in perpendicular and parallel More about this image found in Fluorescence anisotropy reports homo-FRET between cholesterol sensors. (A) ...

Figure 5. Effects of manipulating cholesterol on the equilibrium and kinetic properties of HCN channel gating in nociceptor DRG neurons. (A) Application of WSC decreased the current amplitude of HCN currents in the same-patch experiment. More about this image found in Effects of manipulating cholesterol on the equilibrium and kinetic properti...

Figure 6. Effects of cholesterol enrichment on HCN channel function in nociceptor DRG neurons from naïve and SNI animals. (A) Representative protocol and membrane potential recordings using hyperpolarizing current injection (−90 pA) to activate More about this image found in Effects of cholesterol enrichment on HCN channel function in nociceptor DRG...

Figure 1. PDM of hCNGA1 channel concatenated with a genetically encoded photosensitizer, SOPP3. (A) Schematic models illustrating the construction of the hCNGA1-SOPP3 fusion channel (left) and the experimental configuration (right). PDM requires More about this image found in PDM of hCNGA1 channel concatenated with a genetically encoded photosensitiz...