Active Na transport across frog skin was separated from passive Na movement utilizing urea influx as a measure of passive (shunt) permeability. In this manner, the response of the overall active Na transport system to an applied potential was determined over a range from +200 mV to -100 mV. Active Na transport displays saturation as a function of applied potential, and both the level of saturation and the potential at which it is achieved are functions of the Na concentration in the external solution. The saturation with potential appears to involve a different step in the transport process than the saturation of Na flux as a function of external Na concentration. The observations can be qualitatively described by either a one-barrier or two-barrier model of the Na transport system.
Article|
July 01 1973
Response of the Frog Skin to Steady-State Voltage Clamping : II. The active pathway
Lazaro J. Mandel,
Lazaro J. Mandel
From the Department of Physiology, Yale University School of Medicine, New Haven, Connecticut 06510.
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Peter F. Curran
Peter F. Curran
From the Department of Physiology, Yale University School of Medicine, New Haven, Connecticut 06510.
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Lazaro J. Mandel
From the Department of Physiology, Yale University School of Medicine, New Haven, Connecticut 06510.
Peter F. Curran
From the Department of Physiology, Yale University School of Medicine, New Haven, Connecticut 06510.
Dr. Mandel's present address is the Department of Physiology, Duke University Medical School, Durham, North Carolina 27707.
Received:
November 16 1972
Online ISSN: 1540-7748
Print ISSN: 0022-1295
Copyright © 1973 by The Rockefeller University Press
1973
J Gen Physiol (1973) 62 (1): 1–24.
Article history
Received:
November 16 1972
Citation
Lazaro J. Mandel, Peter F. Curran; Response of the Frog Skin to Steady-State Voltage Clamping : II. The active pathway . J Gen Physiol 1 July 1973; 62 (1): 1–24. doi: https://doi.org/10.1085/jgp.62.1.1
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