Microsomes from rat submaxillary glands are able to take up calcium from the suspension media. Calcium uptake is greatly increased by the presence of ATP. This effect of ATP is not detected at 0°C. ADP cannot replace ATP to potentiate calcium uptake. ATP-dependent calcium uptake is not observed in the absence of magnesium. ATP-dependent calcium uptake is enhanced by oxalate and, to a lesser degree, by inorganic phosphate. Total calcium per milligram of microsomal protein observed when tests were performed without oxalate closely parallels the amounts for skeletal and cardiac muscles reported by several authors. Calcium uptake in salivary gland microsomes is slower than in muscle microsomes. Speculations are considered about the role of ATP-dependent calcium uptake. It is suggested that a decrease in intracellular free calcium levels returns these cells to the resting state after secretion.
Article|
September 01 1971
Adenosine Triphosphate-Dependent Calcium Uptake by Rat Submaxillary Gland Microsomes
G. L. Alonso,
G. L. Alonso
From the Cátedra de Fisiología, Facultad de Odontología de la Universidad de Buenos Aires, Buenos Aires, Argentina
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P. M. Bazerque,
P. M. Bazerque
From the Cátedra de Fisiología, Facultad de Odontología de la Universidad de Buenos Aires, Buenos Aires, Argentina
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D. M. Arrigó,
D. M. Arrigó
From the Cátedra de Fisiología, Facultad de Odontología de la Universidad de Buenos Aires, Buenos Aires, Argentina
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O. R. Tumilasci
O. R. Tumilasci
From the Cátedra de Fisiología, Facultad de Odontología de la Universidad de Buenos Aires, Buenos Aires, Argentina
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G. L. Alonso
From the Cátedra de Fisiología, Facultad de Odontología de la Universidad de Buenos Aires, Buenos Aires, Argentina
P. M. Bazerque
From the Cátedra de Fisiología, Facultad de Odontología de la Universidad de Buenos Aires, Buenos Aires, Argentina
D. M. Arrigó
From the Cátedra de Fisiología, Facultad de Odontología de la Universidad de Buenos Aires, Buenos Aires, Argentina
O. R. Tumilasci
From the Cátedra de Fisiología, Facultad de Odontología de la Universidad de Buenos Aires, Buenos Aires, Argentina
Received:
March 26 1971
Online Issn: 1540-7748
Print Issn: 0022-1295
Copyright © 1971 by The Rockefeller University Press
1971
J Gen Physiol (1971) 58 (3): 340–350.
Article history
Received:
March 26 1971
Citation
G. L. Alonso, P. M. Bazerque, D. M. Arrigó, O. R. Tumilasci; Adenosine Triphosphate-Dependent Calcium Uptake by Rat Submaxillary Gland Microsomes . J Gen Physiol 1 September 1971; 58 (3): 340–350. doi: https://doi.org/10.1085/jgp.58.3.340
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