These studies have developed and tested an experimental approach to the study of membrane ionic conductance mechanisms in strips of uterine smooth muscle. The experimental and theoretical basis for applying the double sucrose-gap technique is described along with the limitations of this system. Nonpropagating membrane action potentials were produced in response to depolarizing current pulses under current-clamp conditions. The stepwise change of membrane potential under voltage-clamp conditions resulted in a family of ionic currents with voltage- and time-dependent characteristics. In sodium-free solution the peak transient current decreased and its equilibrium potential shifted along the voltage axis toward a more negative internal potential. These studies indicate a sodium-dependent, regenerative excitation mechanism.
Article| August 01 1969
Voltage-Clamp Studies on Uterine Smooth Muscle
Nels C. Anderson, Jr.
From the Departments of Obstetrics and Gynecology, and the Department of Physiology and Pharmacology, Duke University Medical Center, Durham, North Carolina 27706
Received: February 27 1969
Online Issn: 1540-7748
Print Issn: 0022-1295
Copyright © 1969 by The Rockefeller University Press
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Nels C. Anderson; Voltage-Clamp Studies on Uterine Smooth Muscle . J Gen Physiol 1 August 1969; 54 (2): 145–165. doi: https://doi.org/10.1085/jgp.54.2.145
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