Several properties of the enzyme acetylcholinesterase (AChE) isolated in vitro are compared with those of the membrane receptor(s) of acetylcholine expressed by the in vivo electrical response of the electroplax membrane. AChE strongly binds in vitro effectors of the electroplax: agonists e.g., decamethonium or antagonists, e.g., d-tubocurarine and flaxedil. It also reacts covalently with an affinity labeling reagent of the acetylcholine receptor site(s) in vivo (TDF). Two classes of sites on AChE molecule account for the binding of these quaternary nitrogen containing compounds: (1) the anionic site of the active center and (2) noncatalytic "peripheral anionic centers" located outside the active center. A disulfide bond breaking agent, dithiothreitol (DTT) alters in a parallel manner the reaction of AChE and the excitable membrane of the electroplax to TDF. The irreversibility of TDF action is lost in both cases, after exposure to DTT. Both AChE and the acetylcholine receptor thus contain disulfide bonds—they are closely related but not necessarily identical proteins.
Article|
July 01 1969
On Some Structural Analogies between Acetylcholinesterase and the Macromolecular Receptor of Acetylcholine
Jean-Pierre Changeux,
Jean-Pierre Changeux
From the Service de Biochimie Cellulaire, Institut Pasteur, Paris, France
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Thomas Podleski,
Thomas Podleski
From the Service de Biochimie Cellulaire, Institut Pasteur, Paris, France
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Jean-Claude Meunier
Jean-Claude Meunier
From the Service de Biochimie Cellulaire, Institut Pasteur, Paris, France
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Jean-Pierre Changeux
From the Service de Biochimie Cellulaire, Institut Pasteur, Paris, France
Thomas Podleski
From the Service de Biochimie Cellulaire, Institut Pasteur, Paris, France
Jean-Claude Meunier
From the Service de Biochimie Cellulaire, Institut Pasteur, Paris, France
Online ISSN: 1540-7748
Print ISSN: 0022-1295
Copyright © 1969 by The Rockefeller University Press
1969
J Gen Physiol (1969) 54 (1): 225–244.
Citation
Jean-Pierre Changeux, Thomas Podleski, Jean-Claude Meunier; On Some Structural Analogies between Acetylcholinesterase and the Macromolecular Receptor of Acetylcholine . J Gen Physiol 1 July 1969; 54 (1): 225–244. doi: https://doi.org/10.1085/jgp.54.1.225
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