1. Chemical examination of the cell sap of Nitella showed that the concentrations of all the principal inorganic elements, K, SO4, Ca, Mg, PO4, Cl, and Na, were very much higher than in the water in which the plants were growing.
2. Conductivity measurements and other considerations lead to the conclusion that all or nearly all of the inorganic elements present in the cell sap exist in ionic state.
3. The insoluble or combined elements found in the cell wall or protoplasm included Ca, Mg, S, Si, Fe, and Al. No potassium was present in insoluble form. Calcium was predominant.
4. The hydrogen ion concentration of healthy cells was found to be approximately constant, at pH 5.2. This value was not changed even when the outside solution varied from pH 5.0 to 9.0.
5. The penetration of NO3 ion into the cell sap from dilute solutions was definitely influenced by the hydrogen ion concentration of the solution. Penetration was much more rapid from a slightly acid solution than from an alkaline one. It is possible that the NO3 forms a combination with some constituent of the cell wall or of the protoplasm.
6. The exosmosis of chlorine from Nitella cells was found to be a delicate test for injury or altered permeability.
7. Dilute solutions of ammonium salts caused the reaction of the cell sap to increase its pH value. This change was accompanied by injury and exosmosis of chlorine.
8. Apparently the penetration of ions into the cell may take place from a solution of low concentration into a solution of higher concentration.
9. Various comparisons with higher plants are drawn, with reference to buffer systems, solubility of potassium, removal of nitrate from solution, etc.