It is shown (1) that Valonia and Halicystis cells exhibit varying degrees of tolerance to injections of animal peptone, animal proteose, crystallized egg albumen, and diphtheria toxin; (2) that Valonia cells display decreased tolerance to egg albumen in increasing dosages, although Halicystis is completely tolerant of the highest dosage used; (3) that the mortality curves of Valonia injected with egg albumen and of both Valonia and Halicystis injected with diphtheria toxin show the delayed effect characteristic of laboratory mammals when treated similarly; (4) that Valonia cells injected twice with egg albumen exhibit no change in susceptibility to its effects; and (5) that neither species of algae gives evidence of having formed antibodies against the antigens used.

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