![Figure 5. Reconstruction of t-BuBHQ-induced [Ca2+]i transients and their modification by caffeine and ryanodine. Simulated effects of sudden inhibition of ER Ca2+ uptake on ci (∼[Ca2+]i, A), JRelease (B), and intraluminal Ca2+ concentration, cER (∼\batchmode \documentclass[fleqn,10pt,legalpaper]{article} \usepackage{amssymb} \usepackage{amsfonts} \usepackage{amsmath} \pagestyle{empty} \begin{document} \(\mathrm{{\Delta}}\left[\mathrm{Ca}^{2\mathrm{+}}\right]_{\mathrm{ER}}^{\left(\mathrm{i}\right)}\left(\mathrm{t}\right)\) \end{document}). Simulations were performed as described in appendix B using experimentally determined descriptions of κi from Fig. 1 D, JSERCA from Fig. 2 C, and PER(vi/vERκER) from Fig. 4 D. Jpm was described by smooth curves obtained from analysis of individual cells from Fig. 4 as in Fig. 3 C, with initial values of ci based on estimates of resting [Ca2+]i in those same cells. Insets in A and B show simulations performed assuming a fixed initial value (50 nM) for ci.](https://cdn.rupress.org/rup/content_public/journal/jgp/119/4/10.1085_jgp.20020315119211/7/m_jgp1194c1f5.jpeg?Expires=1729314048&Signature=qlSHRpw2AA1uwWElhQhgx1v9L3K00HprxynSLm-ihTwl97Zj~Tl1g6IDQP-zTr9xL4InYwYVHhhsJcH0WEf1Bs4GpelhI1T4V0pWRKXfwj~R5SK73hQCIk9ofweEdxI23-E7cb6uLcB304HpOhBKZfpnqAvTQoIVe3r2Cp~pyEJ-e4~h~zxRpXnBaBs5a9m6h2-YWwjqcLGE7N1tBAxRwk8Y6HWeQvGKjuYvM~fltQnNYWJu4TTNG0L-Fm6v1JcQn7-trVQam-2rnesNmDuqEj1Al4yhTNHcO3t-gJAcPX0WrdGMzzLhVrErZondKANfnhv7GNvvbeBRlWEZhEvIIQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Reconstruction of t-BuBHQ-induced [Ca2+]i transients and their modification by caffeine and ryanodine. Simulated effects of sudden inhibition of ER Ca2+ uptake on ci (∼[Ca2+]i, A), JRelease (B), and intraluminal Ca2+ concentration, cER (∼ \(\mathrm{{\Delta}}\left[\mathrm{Ca}^{2\mathrm{+}}\right]_{\mathrm{ER}}^{\left(\mathrm{i}\right)}\left(\mathrm{t}\right)\) ). Simulations were performed as described in appendix B using experimentally determined descriptions of κi from Fig. 1 D, JSERCA from Fig. 2 C, and PER(vi/vERκER) from Fig. 4 D. Jpm was described by smooth curves obtained from analysis of individual cells from Fig. 4 as in Fig. 3 C, with initial values of ci based on estimates of resting [Ca2+]i in those same cells. Insets in A and B show simulations performed assuming a fixed initial value (50 nM) for ci.
Reconstruction of t-BuBHQ-induced [Ca2+]i transients and their modification by caffeine and ryanodine. Simulated effects of sudden inhibition of ER Ca2+ uptake on ci (∼[Ca2+]i, A), JRelease (B), and intraluminal Ca2+ concentration, cER (∼ \(\mathrm{{\Delta}}\left[\mathrm{Ca}^{2\mathrm{+}}\right]_{\mathrm{ER}}^{\left(\mathrm{i}\right)}\left(\mathrm{t}\right)\) ). Simulations were performed as described in appendix B using experimentally determined descriptions of κi from Fig. 1 D, JSERCA from Fig. 2 C, and PER(vi/vERκER) from Fig. 4 D. Jpm was described by smooth curves obtained from analysis of individual cells from Fig. 4 as in Fig. 3 C, with initial values of ci based on estimates of resting [Ca2+]i in those same cells. Insets in A and B show simulations performed assuming a fixed initial value (50 nM) for ci.
