1. By a procedure involving the use of acid acetone hemoglobin may be rapidly separated into a precipitate of denatured globin and an acetone solution of heme.
2. By neutralization procedures the denatured globin may be largely converted into a soluble, apparently native form which can combine with heme to form hemoglobin.
3. The heme may be obtained in acetone-free, slightly alkaline solution without the use of strong alkali which may modify the heme.
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Copyright, 1930, by The Rockefeller Institute for Medical Research
1930
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