The electrophysiological properties of embryonic chick hearts (ventricles) change during development; the largest changes occur between days 2 and 8. Resting potential (Em) and peak overshoot potential (+Emax) increase, respectively, from -35 mv and +11 mv at day 2 to -70 mv and +28 mv at days 12–21. Action potential duration does not change significantly. Maximum rate of rise of the action potential (+Vmax) increases from about 20 v/sec at days 2–3 to 150 v/sec at days 18–21; + Vmax of young cells is not greatly increased by applied hyperpolarizing current pulses. In resting Em vs. log [K+]o curves, the slope at high K+ is lower in young hearts (e.g. 30 mv/decade) than the 50–60 mv/decade obtained in old hearts, but the extrapolated [K+]i values (125–140 mM) are almost as high. Input resistance is much higher in young hearts (13 MΩ at day 2 vs. 4.5 MΩ at days 8–21), suggesting that the membrane resistivity (Rm) is higher. The ratio of permeabilities, PNa/PK, is high (about 0.2) in young hearts, due to a low PK, and decreases during ontogeny (to about 0.05). The low K+ conductance (gK) in young hearts accounts for the greater incidence of hyperpolarizing afterpotentials and pacemaker potentials, the lower sensitivity (with respect to loss of excitability) to elevation of [K+]o, and the higher chronaxie. Acetylcholine does not increase gK of young or old ventricular cells. The increase in (Na+, K+)-adenosine triphosphatase (ATPase) activity during development tends to compensate for the increase in gK. +Emax and + Vmax are dependent on [Na+]o in both young and old hearts. However, the Na+ channels in young hearts (2–4 days) are slow, tetrodotoxin (TTX)-insensitive, and activated-inactivated at lower Em. In contrast, the Na+ channels of cells in older hearts (> 8 days) are fast and TTX-sensitive, but they revert back to slow channels when placed in culture.
Article|
October 01 1972
Changes in Membrane Properties of Chick Embryonic Hearts during Development
Nick Sperelakis,
Nick Sperelakis
From the Department of Physiology, University of Virginia School of Medicine, Charlottesville, Virginia 22903
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K. Shigenobu
K. Shigenobu
From the Department of Physiology, University of Virginia School of Medicine, Charlottesville, Virginia 22903
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Nick Sperelakis
From the Department of Physiology, University of Virginia School of Medicine, Charlottesville, Virginia 22903
K. Shigenobu
From the Department of Physiology, University of Virginia School of Medicine, Charlottesville, Virginia 22903
Received:
January 05 1972
Online ISSN: 1540-7748
Print ISSN: 0022-1295
Copyright © 1972 by The Rockefeller University Press
1972
J Gen Physiol (1972) 60 (4): 430–453.
Article history
Received:
January 05 1972
Citation
Nick Sperelakis, K. Shigenobu; Changes in Membrane Properties of Chick Embryonic Hearts during Development . J Gen Physiol 1 October 1972; 60 (4): 430–453. doi: https://doi.org/10.1085/jgp.60.4.430
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