Nitrous Acid Reactivation of Ultraviolet-Irradiated Transforming DNA from Hemophilus influenzae

Cabrera-Juárez , Emiliano

doi:10.1085/jgp.47.2.279

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Article| November 01 1963

Nitrous Acid Reactivation of Ultraviolet-Irradiated Transforming DNA from Hemophilus influenzae

Emiliano Cabrera-Juárez

From the Department of Biochemistry, The Johns Hopkins University School of Hygiene and Public Health, Baltimore

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Author and Article Information

Emiliano Cabrera-Juárez

From the Department of Biochemistry, The Johns Hopkins University School of Hygiene and Public Health, Baltimore

Received: March 29 1963

Online ISSN: 1540-7748

Print ISSN: 0022-1295

1963

J Gen Physiol (1963) 47 (2): 279–296.

https://doi.org/10.1085/jgp.47.2.279

Partial recovery of ultraviolet-damaged denatured or native transforming DNA from Hemophilus influenzae, has been obtained by exposing the irradiated DNA in the denatured form to nitrous acid. Some factors that affect this recovery are described. An erythromycin marker (E₂₀) was not reactivated. The UV damage reactivable by nitrous acid is different from that repaired by the photoreactivating enzyme from bakers' yeast. The pretreatment with nitrous acid affords a slight protection for denatured C₂₅ DNA and Sm₂₅₀ DNA against ultraviolet irradiation, but this pretreatment sensitized the E₂₀ DNA to this irradiation.

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