The respiration of Chlorella is diminished by exposure to hypertonic salt solutions. After a short exposure there is complete recovery when the algæ are removed to the normal medium. After a longer exposure recovery may be incomplete, as shown by the fact that the rate of respiration fails to rise to the normal level. Staining with methylene blue indicates that few, if any, of the cells are killed as the result of the exposure. It would therefore seem that the treatment produces a persistent lowering of the rate of metabolism. Such a condition of metabolism is also found after exposure to chloroform.

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