If we increase the osmotic pressure at one end of a Nitella cell by applying a solution of sucrose and if we subsequently submerge the entire cell in water we find that water enters at the end where the osmotic pressure is higher and comes out of the cell at the other end.

If similar inequalities of osmotic pressure should arise as the result of metabolism we can understand how a secreting cell might take up water at one spot on its surface and expel it in another spot and thus bring about the secretion of water.

The Nitella cell can expel water from a region of the cell which is in contact with water, air, or mineral oil.

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