1. The globin prepared from hemoglobin by the acid acetone method is denatured globin.
2. The denaturation and coagulation of globin by acid acetone are reversible.
3. Soluble globin can be obtained from the acid acetone globin even if the globin is first precipitated by trichloracetic acid or heated to 100°C.
4. Hill and Holden's theory that they separated native globin from hemoglobin without any intermediate denaturation is not proven by their experiments.
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Copyright, 1931, by The Rockefeller Institute for Medical Research
1931
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