When MnCl2 is injected into the cells of Valonia macrophysa they live only about half as long as when the same concentration is applied to the exterior of the cell. This is due to toxic action and not to the mechanical disturbance accompanying the injection (since all cells were stabbed in the same manner by the capillary).

A variety of explanations are suggested, all of which involve a difference between the inner and outer layers of the protoplasm.

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