ClC-type anion-selective channels are widespread throughout eukaryotic organisms. BLAST homology searches reveal that many microbial genomes also contain members of the ClC family. An Escherichia coli–derived ClC Cl− channel homologue, “EriC,” the product of the yadQ gene, was overexpressed in E. coli and purified in milligram quantities in a single-step procedure. Reconstitution of purified EriC into liposomes confers on these membranes permeability to anions with selectivity similar to that observed electrophysiologically in mammalian ClC channels. Cross-linking studies argue that EriC is a homodimer in both detergent micelles and reconstituted liposomes, a conclusion corroborated by gel filtration and analytical sedimentation experiments.
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1 November 1999
Article|
October 25 1999
High-Level Expression, Functional Reconstitution, and Quaternary Structure of a Prokaryotic Clc-Type Chloride Channel
Merritt Maduke,
Merritt Maduke
aFrom the Department of Biochemistry, Howard Hughes Medical Institute, Brandeis University, Waltham, Massachusetts 02254-9110
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Deborah J. Pheasant,
Deborah J. Pheasant
aFrom the Department of Biochemistry, Howard Hughes Medical Institute, Brandeis University, Waltham, Massachusetts 02254-9110
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Christopher Miller
Christopher Miller
aFrom the Department of Biochemistry, Howard Hughes Medical Institute, Brandeis University, Waltham, Massachusetts 02254-9110
Search for other works by this author on:
Merritt Maduke
aFrom the Department of Biochemistry, Howard Hughes Medical Institute, Brandeis University, Waltham, Massachusetts 02254-9110
Deborah J. Pheasant
aFrom the Department of Biochemistry, Howard Hughes Medical Institute, Brandeis University, Waltham, Massachusetts 02254-9110
Christopher Miller
aFrom the Department of Biochemistry, Howard Hughes Medical Institute, Brandeis University, Waltham, Massachusetts 02254-9110
1used in this paper: DDM, dodecylmaltoside; MES, morpholino ethanesulfonic acid; SDS, sodium dodecylsulfate
Received:
July 29 1999
Revision Requested:
September 20 1999
Accepted:
September 27 1999
Online ISSN: 1540-7748
Print ISSN: 0022-1295
© 1999 The Rockefeller University Press
1999
The Rockefeller University Press
J Gen Physiol (1999) 114 (5): 713–722.
Article history
Received:
July 29 1999
Revision Requested:
September 20 1999
Accepted:
September 27 1999
Citation
Merritt Maduke, Deborah J. Pheasant, Christopher Miller; High-Level Expression, Functional Reconstitution, and Quaternary Structure of a Prokaryotic Clc-Type Chloride Channel. J Gen Physiol 1 November 1999; 114 (5): 713–722. doi: https://doi.org/10.1085/jgp.114.5.713
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