1. Imaging beyond the Diffraction Limit JAY K. TRAUTMAN, Praelux, Incorporated, Lawrenceville, New Jersey
2. Dynamic Views of Living Cell Fine Structure Revealed by Electro-optically Modulated Polarization Microscopy RUDOLF OLDENBOURG, Marine Biological Laboratory, Woods Hole, Massachusetts 02543
We have been developing and applying a new type of polarized light microscope (the “Pol-Scope”) that dramatically enhances the unique capabilities of the traditional polarizing microscope. The Pol-Scope incorporates a universal compensator, made from computer-driven liquid crystal devices, to measure the birefringent fine structure simultaneously over the whole field of view for all orientations of the birefringence axis, at high sensitivity (0.02 nm birefringence retardation), high resolution (0.2 μm), and in fast time intervals (1 s). The new Pol-Scope reveals cellular fine structure without having to treat the cell with exogenous dyes or fluorescent labels. Pol-Scope images provide quantitative information about intrinsic optical parameters that can...
