Search Results for Corona

Figure 1. Fibrous corona expansion requires CENP-E. (A) Immunostaining of ZWILCH (magenta), CENP-E (green), and CENP-C (blue) in control and CENP-E–depleted RPE1 cells treated with nocodazole overnight. Bar, 5 μm; inset bar, 1 μm. (B) Immunostaining of ZWILCH (magenta), SPINDLY (green More about this image found in Fibrous corona expansion requires CENP-E. (A) Immunostaining of ZWILCH (ma...

Figure 2. Fibrous corona formation requires C-terminal farnesylation of CENP-E. (A) Domain organization of CENP-E 2111-C and various respective mutants. 2111-C fragment of CENP-E with different stretches of 10 amino acids were substituted for alanine residues or was deleted of the C-terminal 12 More about this image found in Fibrous corona formation requires C-terminal farnesylation of CENP-E. (A) ...

Figure 5. Farnesylation promotes interaction of endogenous CENP-E with fibrous corona proteins in mitosis. (A) Immunostaining of ZW10 (green), CENP-E (magenta), and CENP-C (blue) in STLC-treated RPE1 cells cotreated with DMSO or GSK923295 overnight. Bar, 5 μm; inset bar, 1 μm. (B More about this image found in Farnesylation promotes interaction of endogenous CENP-E with fibrous corona...

Figure 6. CENP-E impacts fibrous corona formation after initial RZZ-S kinetochore recruitment. (A) Immunostaining of ZW10 (green), CENP-E (magenta), and CENP-C (blue) in RPE1 cells treated with nocodazole for indicated time, which had been arrested with STLC and GSK923295 overnight. Bar, 5 μm More about this image found in CENP-E impacts fibrous corona formation after initial RZZ-S kinetochore rec...

Figure 7. Comparative genomics of fibrous corona formation mechanisms in eukaryotes. Phylogenetic profiling of CENP-E, the CAAX box of CENP-E, SPINDLY, the CAAX box of SPINDLY, ROD, ZWILCH, and ZW10 across eukaryotic diversity represented by selected species. Evolutionary reconstructions More about this image found in Comparative genomics of fibrous corona formation mechanisms in eukaryotes. ...

Figure 3. CENP-F controls corona localization in a microtubule-dependent manner. (a) Immunofluorescence microscopy images of early prometaphase HeLa-K, CNP-F-Mut1, and CENP-F-Mut2 cells stained with DAPI and antibodies against CENP-C and CENP-E. Scale bar, 5 µm. (b) Quantification More about this image found in CENP-F controls corona localization in a microtubule-dependent manner. (a) ...

Figure 5. CENP-F functions to limit dynein stripping of the corona. (a) Left: Immunofluorescence microscopy images from the dynein-stripping assay for CENP-E in CENP-F-Mut1 cells. Cells were treated with control or DHC siRNA, arrested in nocodazole or monastrol, and stained with DAPI More about this image found in CENP-F functions to limit dynein stripping of the corona. (a) Left: Immuno...

Figure 8. CENP-F-Nde1 controls corona stripping by dynein in untreated cells. (a) Immunofluorescence microscopy images of HeLa-K, CENP-F-Mut1, and CENP-F-Mut2 cells transfected with an empty vector, eGFP-CENP-F(2021–2901), or eGFP-CENP-F(2351–2901) and stained with DAPI and antibodies against More about this image found in CENP-F-Nde1 controls corona stripping by dynein in untreated cells. (a) Im...

Figure 9. CENP-F-Nde1 controls corona stripping by dynein the nocodazole-monastrol assay. (a) Immunofluorescence microscopy images of the nocodazole-monastrol dynein-stripping assay in CENP-F-Mut1 and CENP-F-Mut2 cells transfected with an empty vector, eGFP-CENP-F(2021–2901), or eGFP-CENP-F More about this image found in CENP-F-Nde1 controls corona stripping by dynein the nocodazole-monastrol as...

Figure 6 CENP-E is a integral component of kinetochore corona fibers extending 50 nm from the outer plate. HeLa cells were initially treated with a low dose of nocodazole for 12 h to disassemble microtubules and processed as described in Fig. 2 . Nocodazole treatment bulges the kinetochore More about this image found in CENP-E is a integral component of kinetochore corona fibers extending 50 nm...

Figure 1. The Spindly–dynein–dynactin complex binds to the RZZ complex and initiates the disassembly of the fibrous corona and removal of checkpoint proteins upon kinetochore–microtubule attachment. (A) At unattached kinetochores, binding of the KMN network to microtubules is inhibited More about this image found in The Spindly–dynein–dynactin complex binds to the RZZ complex and initiates ...

image (out of three splenic samples analyzed) of a GC surrounded by densely distributed IgD⁺CD27⁻ naive cells forming the corona and the MZ at the periphery, occupied by CD27⁺IgD^low B cells (lightly colored in purple; splenic section of a 4-yr-old child; bar More about this image found in Non-lymphoid DLL1⁺ cells are located in the splenic MZ. Confoca...

. As part of the fibrous corona, DYNEIN-DYNACTIN-SPINDLY assists in chromosome movement. Later in metaphase, the motor complex also contributes to corona disassembly by promoting RZZ removal from kinetochores. More about this image found in Conformational changes in SPINDLY control DYNEIN activation at kinetochores...

of another chromosome (more clearly seen in adjacent sections). (C) Higher magnification view shows that CENP-E is located to the corona fibers of the kinetochore. op, outer plate; ip, inner plate; cf, corona fibers. Bars: (A) 2 μm; (B) 170 nm More about this image found in At metaphase CENP-E extends from the kinetochore outer plate at least 50 nm...