Panel A shows the full range of occupancies from 0 to 1. The x-axis represents time in milliseconds, ranging from 0 to 50 milliseconds. The y-axis represents occupancy, ranging from 0 to 1. The graph includes states labeled A3F asterisk, A2F asterisk, P open, R, AR, and A2R. Solid lines represent triliganded states, dashed lines represent diliganded states, and dashed-dot lines represent monoliganded states. The resting conformation is plotted in black, the flipped conformation in green, and the open conformation in red. The total open probability is shown as a solid orange line. Panel B shows an enlarged view of occupancies below 0.1. The x-axis represents time in milliseconds, ranging from 0 to 50 milliseconds. The y-axis represents occupancy, ranging from 0 to 0.1. The graph includes states labeled A3F, A2F asterisk, A2F, A2R, and AF. The same color and line schemes are used as in Panel A.
The time course of occupancies. The time course of glycine receptor state occupancies is shown over the full range, 0–1, in A, and as an enlarged view of occupancies below 0.1 in B. The occupancies were calculated from Eq. 1 and plotted for all 10 states of the flip mechanism (Fig. 3) after a step in glycine concentration from 0 to 1 mM, and, 10 ms later, a step from 1 mM to zero (the concentration profile is shown on top of panel A as a black solid line).The predicted occupancies were calculated, as in Figs. 7 and 8, using the rate constants from one of the three sets of observations fromBurzomato et al. (2004; the set that was used for the illustrations in that paper), not the averaged values shown in Fig. 3. Occupancies of the resting conformation are plotted in black, the flipped conformation is plotted in green, and the open conformation is plotted in red. Solid lines represent triliganded states, dashed lines those of diliganded states, and dashed-dot lines show monoliganded states. Occupancy of the unliganded resting state R (state 10) is plotted as a dotted black line and the total open probability as a solid orange line.
