Panel A shows a schematic explanation of cell categories under study. Panel B presents box plots of G1 lengths for different rounds of daughter cells of G1-switchers and S/G2/M-switchers, with steady-state phases on the outermost left and right. The y-axis represents G1 length in minutes, and the x-axis categorizes the rounds of daughters. Panel C displays box plots of cell volume at the end of G1 for the same cell categories and conditions as in Panel B. The y-axis represents volume in femtoliters (fL), and the x-axis categorizes the rounds of daughters. Both panels include data for wild type (WT), whi5, bck2, and whi5 bck2 cells, with statistical annotations indicating significance levels.
Cells that face the nutrient switch as buds arrest in their first G1, leading to stronger enlargement in bck2Δ cells. (A) Schematic explanation for cell categories analyzed in this figure. (B) G1 lengths are plotted for the rounds of daughters (blue) of G1-switchers and S/G2/M-switchers. The outermost left and right panels show steady-state phase lengths for SCD and SCGE, respectively. For each growth medium, complete cell cycles of daughter cells were pooled from two independent steady-state experiments. (C) Same dataset was also used to determine cell volume at the end of the G1 (fL). The number of cells in each box in the steady-state panels is between 198 and 330. For the nutrient switch panels (blue), cells were pooled from three independent experiments. For the first round of daughters of G1-switchers: nWT = 103, nwhi5Δ = 21, nbck2Δ = 48, nwhi5Δbck2Δ = 33. For the first round of daughters of S/G2/M-switchers: nWT = 123, nwhi5Δ = 40, nbck2Δ = 34, nwhi5Δbck2Δ = 50. The next rounds of daughters had fewer cells than the first round. If a category includes < 5 cells, the individual data points are shown. Two-sided Mann–Whitney U tests were used for statistical analyses (***: P < 0.001; **: P < 0.01; ns: not significant, P ≥ 0.05). For boxplots in B and C, box limits show the IQR, whiskers extend to the most extreme data points within 1.5× IQR, the median is shown as a line, and the mean is shown as a cross. WT, wild type; IQR, interquartile range.