Figure 3.
Panel A shows a flowchart depicting the live-cell imaging analysis pipeline. It starts with time-lapse live-cell imaging, followed by image analysis with Cell-ACDC, which includes automated cell segmentation and tracking with manual correction, and semi-automated pedigree annotation. The final dataset consists of approximately 330 videos containing around 11,000 complete cell cycles. Panel B shows a series of time-lapse live cell images showing wild-type cells during a nutrient switch experiment. The images are taken at different time points, starting with glucose and switching to glycerol-ethanol. The time points are marked in hours, and the nutrient switch occurs at the 2-hour mark. Panel C shows a diagram categorizing cells of interest for downstream analysis of the nutrient switch live-cell microscopy dataset. The categories include G1-switchers, S/G2/M-switchers, and switch cell cycles, with subsequent rounds of daughters.
Analysis strategy for live-cell microscopy of the adaptation to the nutrient switch. (A) Live-cell imaging analysis pipeline for steady-state and nutrient switch experiments. Cell cycle stage is classified as G1 (unbudded cells) or S/G2/M (budded cells). (B) Representative images of WT cells in the nutrient switch live-cell microscopy experiment. (C) Cell categories of interest in the downstream analysis of the nutrient switch live-cell microscopy dataset. Scale bars represent 20 µm. WT, wild type.