Panel A shows a line graph of calcium ion transients in LVCM, comparing control and SMTC conditions; the x-axis represents time, and the y-axis represents F360/F380. Panel B shows a line graph of calcium ion transients in RVCM, comparing control and SMTC conditions; the x-axis represents time, and the y-axis represents F360/F380. Panel C shows a bar graph of resting F360/F380 in LVCM and RVCM under control and SMTC conditions; the y-axis represents F360/F380 at rest. Panel D shows a bar graph of peak F360/F380 in LVCM and RVCM under control and SMTC conditions; the y-axis represents F360/F380 at peak. Panel E shows a bar graph of change in F360/F380 at peak in LVCM and RVCM under control and SMTC conditions; the y-axis represents ΔF360/F380 at peak. Panel F shows a bar graph of time to 10 percent decay in LVCM and RVCM under control and SMTC conditions; the y-axis represents time in milliseconds. Panel G shows a bar graph of time to 50 percent decay in LVCM and RVCM under control and SMTC conditions; the y-axis represents time in milliseconds. Panel H shows a bar graph of time to 90 percent decay in LVCM and RVCM under control and SMTC conditions; the y-axis represents time in milliseconds.
Effects of nNOS inhibition on Ca 2+ transients in LVCMs and RVCMs. (A) Representative intracellular Ca2+ transient traces recorded as F360/F380 in LVCMs under control conditions and following inhibition of nNOS with SMTC. Solid black line, LVCM; black dotted line, LVCM + SMTC. The timing of electrical stimulation and peak Ca2+ signal are indicated. (B) Representative Ca2+ transient traces from RVCMs under control conditions and following SMTC treatment. Solid gray line, RVCM; gray dotted line, RVCM + SMTC. (C) Resting Ca2+ signal expressed as F360/F380 was measured. (D) Peak Ca2+ signal (F360/F380 at peak) measured in each experimental group. (E) Ca2+ transient amplitude expressed as ΔF360/F380 at peak. (F–H) Time to 10% (F), 50% (G), and 90% (H) decay of the Ca2+ transient following peak. In panels C–H, gray dots represent individual cardiomyocytes, whereas black dots represent the mean value for each animal. Sample sizes were as follows (N = 10 animals per group): LVCM, n = 103; LVCM + SMTC, n = 201; RVCM, n = 127; RVCM + SMTC, n = 136. n.s., not significant.