Panel A shows a Western blot with nNOS, p-nNOS at Ser147, and GAPDH in LVCM and RVCM. Panel B shows a bar graph of nNOS expression in LVCM and RVCM, with the y-axis representing expression in arbitrary units. Panel C shows a bar graph of p-nNOS expression in LVCM and RVCM, with the y-axis representing expression in arbitrary units. Panel D shows a bar graph of the ratio of p-nNOS to nNOS in LVCM and RVCM. Panel E shows a bar graph of nitrite concentration in LVCM and RVCM under control, L-NAME, and SMTC conditions, with the y-axis representing nitrite in nanomoles per microgram.
Similar levels of total nNOS expression and NO production between LVCMs and RVCMs. (A) Representative immunoblots showing total nNOS, p-nNOS (p-nNOSSer1417), and GAPDH in LVCMs and RVCMs. (B) Quantification of total nNOS expression normalized to GAPDH in LVCMs (N = 10) and RVCMs (N = 10). (C) Quantification of p-nNOSSer1417 expression normalized to GAPDH in LVCMs and RVCMs. (D) Ratio of p-nNOSSer1417 to total nNOS in LVCMs and RVCMs. No significant differences were detected between groups. (E) Nitrite content measured in LVCMs and RVCMs under control conditions (C) and following inhibition of nNOS with L-NAME (L) or SMTC (S). Both inhibitors significantly reduced nitrite levels in LVCMs and RVCMs compared with control, with no significant difference between ventricular cell types (N = 9). Each dot represents one biological sample from an individual animal, and one sample per animal per condition was included in the quantitative analysis. n.s., not significant. Source data are available for this figure: SourceData F1.