Figure S6.
Panel A shows a line graph with relative current on the y-axis and time in minutes on the x-axis for wild type (8A/8C). The graph illustrates a decrease in relative current during DCPIB application and a gradual recovery during washout. Panel B presents two line graphs comparing wild type (8A/8C) and E6A/E6A mutants. Both graphs show relative current on the y-axis and time in minutes on the x-axis. The wild type data is shown in gray, while the E6A/E6A data is in purple. Panel C displays two line graphs comparing wild type (8A/8C) and L134K/L136K mutants. The wild type data is in gray, and the L134K/L136K data is in yellow. Both graphs show relative current on the y-axis and time in minutes on the x-axis. The trends indicate different recovery patterns during washout for each mutant type.
Kinetics of washin and washout of DCPIB in heteromeric 8A/8C channels. (A–C) Time courses of drug washout in WT (A), E6A/E6A (B), and L134K/L136K (C) LRRC8A/LRRC8C heteromers. WT data (gray line) from A is shown for comparison (8A/8C n = 5, E6A/E6A n = 5, L134K/L136K n = 4). Data are mean ± SEM.