Figure S5.
Panel A shows a voltage clamp protocol used to assess use dependent block by DCPIB. It includes two sets of traces, one for a 2 second pulse and another for a 5 second pulse. The traces are labeled with voltages of positive 60 millivolts and negative 30 millivolts. The time scale is marked at 1 second intervals, with a voltage scale of 20 millivolts. Panel B presents a line graph showing the mean relative current evoked by a pulse at positive 60 millivolts for 50 milliseconds, applied every 2 seconds or 5 seconds. The x-axis represents time in minutes, and the y-axis represents relative current. The graph includes two data series, one for 2 second pulses represented by open circles and another for 5 second pulses represented by open squares, with error bars indicating the standard error of the mean. The application of DCPIB is marked by a horizontal bar above the graph.
Evaluation of use-dependent inhibition of DCPIB. (A) Voltage-clamp protocol used to assess use-dependent block by DCPIB. (B) Mean ± SEM relative current evoked by a pulse (+60 mV for 50 ms) every 2 or 5 s (n = 3).