Figure S4.
Panel A shows the current for the 8C 8A(IL125) M48K mutant, while Panel B shows the current for the 8C 8A(IL125) M48R mutant. Both graphs have the x-axis labeled time in minutes, ranging from 0 to 5 minutes, and the y-axis labeled current in picoamperes per picofarad, ranging from negative 100 to 200 picoamperes per picofarad. The data points are represented with error bars indicating the standard error of the mean, with n equal to 5 for each mutant. In both panels, the currents are measured at positive 100 millivolts and negative 100 millivolts, shown in different shades of green in Panel A and blue in Panel B. The graphs illustrate the response of the mutants to cell swelling under hypotonic conditions.
Swelling-induced activation of 8C-8A(IL125)-M48 mutant. (A and B) 8C-8A(IL125)-M48K (A) and -M48R (B) mutants are responsive to cell swelling. Transfected cells were voltage-ramped between −100 mV and +100 mV in isotonic solution and then subjected to hypotonic cell swelling. Data are mean ± SEM (n = 5 each).