Figure 6.
Panel A shows a side view of the cryo-EM structure of 8C-8A(IL125) with the solvent-accessible permeation pathway highlighted. Panel B displays representative whole-cell currents for M48D, M48K, and M48R mutants recorded under specific conditions. The scale for all traces is 500 picoamperes and 250 milliseconds. Panel C presents a dose-response curve showing the percentage inhibition of these mutants at different concentrations of DCPIB, with the wild-type curve included for comparison. The data points represent mean values with standard error of the mean.
Analysis of 8C-8A(IL1 25 ) M48 mutations on DCPIB sensitivity. (A) Side view of the cryo-EM structure of 8C-8A(IL125) (PDB ID: 8DXN) along with the solvent-accessible permeation pathway (gray surface) calculated using HOLE (Smart et al., 1996). (B) Representative whole-cell currents of the indicated mutants recorded from a cell dialyzed with normal pipette solution and cell swelling with hypotonic buffer. The scale is the same for all traces. (C) CRC data recorded at +100 mV for the indicated mutants. Fit of WT CRC data (black line) from Fig. 3 C is shown for comparison (8C-8A(IL125) n = 6, M48D n = 4–8, M48K and M48R n = 5 each). Data are mean ± SEM.