Figure S3.
Panel A shows a line graph for the 8C 8A(IL1 25) L136K mutant. The x-axis represents voltage in millivolts, ranging from negative 100 to 100, and the y-axis represents current in picoamperes, ranging from negative 1000 to 1000. The black line represents control conditions, the red line represents the application of 10 micromolar DCPIB, and the gray line represents drug washout. Panel B shows a line graph for the 8C 8A(IL1 25) L136E mutant. The x-axis represents voltage in millivolts, ranging from negative 100 to 100, and the y-axis represents current in picoamperes, ranging from negative 10,000 to 10,000. The black line represents control conditions, the red line represents the application of 10 micromolar DCPIB, and the gray line represents drug washout. Panel C shows a line graph for the 8C 8A(IL1 25) E6A mutant. The x-axis represents voltage in millivolts, ranging from negative 100 to 100, and the y-axis represents current in picoamperes, ranging from negative 1000 to 1000. The black line represents control conditions, the red line represents the application of 10 micromolar DCPIB, and the gray line represents drug washout.
Representative currents of drug washout in the indicated mutants. Ramp currents of the indicated point mutants recorded from a cell dialyzed with normal pipette solution plus cell swelling with hypotonic buffer (black), after bath application of 10 μM DCPIB (red), and drug washout (gray).