Figure 4.
Panel A shows structural views of the extracellular domains of 8C 8A(IL125) with highlighted residues L105, Q106, Y108, and N112. The top view displays the overall structure, while the side view zooms in on the highlighted residues. Panel B presents representative whole cell currents for mutants L105F, L105R, Q106A, Y108A, and N112A recorded from cells dialyzed with normal pipette solution and subjected to hypotonic buffer induced swelling. Each subplot shows current traces with different scales for current in picoamperes and time in milliseconds. Panel C depicts concentration response curves (CRCs) for the indicated mutants recorded at positive 100 millivolts. The x-axis represents the concentration of DCPIB in micromolar on a logarithmic scale, and the y-axis shows the percentage of inhibition. The black line represents the fit of wild type (WT) CRC data for comparison. Data points are shown as mean plus or minus standard error of the mean (SEM), with sample sizes indicated.Top of Form
Analysis of 8C-8A(IL1 25 ) OCS and EL1 mutations on DCPIB sensitivity. (A and B) Top (upper panel) and side (bottom panel) views of the extracellular domains of 8C-8A(IL125) (PDB ID: 8DXN) (B) Representative whole-cell currents of the indicated mutants recorded from a cell dialyzed with normal pipette solution and cell swelling with hypotonic buffer. (C) CRC data recorded at +100 mV for the indicated mutants. Fit of WT CRC data (black line) from Fig. 3 C is shown for comparison. Data are mean ± SEM (8C-8A(IL125) n = 6, mutants n = 5 each).