Figure S2.
Panel A shows a schematic of intracellular dialysis with 30 micromolar DCPIB. Panel B and Panel C display time course data of 8A/8C heteromer and native VRAC currents in HCT 116 cells. The x-axis represents time in minutes, and the y-axis represents current in picoamperes per picofarad. The graphs show data at positive 100 millivolts and negative 100 millivolts. Panel B shows the effect of hypotonic cell swelling and the addition of 10 micromolar DCPIB on 8A/8C heteromer currents. Panel C shows the effect of hypotonic cell swelling and the addition of 10 micromolar DCPIB on native VRAC currents. Both panels indicate changes in current over time under these conditions.
Characteristics of DCPIB-dependent inhibition of 8A/8C heteromer and native VRAC. (A) Intracellular dialysis with 30 µM DCPIB. (B and C) Time course of 8A/8C heteromer and native VRAC currents in HCT-116 cells evoked from cells dialyzed with normal pipette solution including 30 μM DCPIB and hypotonic cell swelling (8A/8C n = 5, native VRAC n = 4).