Figure S1.
Panel A shows a line graph with the x-axis labeled millivolts, ranging from minus 100 to 100, and the y-axis labeled picoamperes, ranging from minus 500 to 1000. The black line represents control conditions, and the red line represents the application of 10 micromolar DCPIB. Panel B displays a similar line graph with the same axes and labels, showing the M139L mutation. Panel C presents another line graph with the x-axis labeled millivolts, ranging from minus 100 to 100, and the y-axis labeled picoamperes, ranging from minus 1000 to 2000, depicting the EL1 mutation. Panel D shows a line graph with the x-axis labeled millivolts, ranging from minus 100 to 100, and the y-axis labeled picoamperes, ranging from minus 500 to 1000, illustrating the L139M mutation. Panel E depicts a line graph with the x-axis labeled millivolts, ranging from minus 100 to 100, and the y-axis labeled picoamperes, ranging from minus 500 to 1500, showing the 8A mutation. Panel F presents a line graph with the x-axis labeled millivolts, ranging from minus 100 to 100, and the y-axis labeled picoamperes, ranging from minus 500 to 1000, illustrating the L137M mutation. Each graph compares the control conditions, represented by a black line, with the application of 10 micromolar DCPIB, represented by a red line.
Representative currents of the swapped point mutations in the TM2. (A–F) Ramp currents of background chimeras and the indicated point mutants recorded from a cell dialyzed with normal (A–D) or low ionic strength (E and F) pipette solution plus cell swelling with hypotonic buffer (black), after bath application of 10 μM DCPIB (red).