Figure 1.
Panel A: A schematic representation of the phagocyte NADPH oxidase complex. The resting state shows components like EROS, gp91phox, p22phox, RAC2, p40, p47, p67, GDI, PKC, and DOCK2. The activated state shows phosphorylation and interaction of these components leading to the production of reactive oxygen species (ROS). Panel B: Four histograms representing DHR assay results for neutrophils. The top left histogram shows a healthy control with non-stimulated and stimulated states. The top right histogram shows a female carrier of a deleterious CYBB variant with non-stimulated and stimulated states. The bottom left histogram shows abolished ROS production, and the bottom right histogram shows impaired ROS production.
NADPH oxidase assembly and the functional DHR assay. (A) Schematic representation of the phagocyte NADPH oxidase complex in the resting (left) and activated (right) states. P, phosphorylation. (B) Schematic representation of DHR assay results for neutrophils showing a healthy control, a female carrier of a deleterious CYBB variant, and abolished and impaired DHR oxidation. NS, non-stimulated.