Figure S3.
Panel A shows representative flow cytometry plots for 30 microliters Ad SenNP immunized mice, with scatter plots for the nasal cavity, BAL, and lung. Each plot displays the frequency of IV positive or IV negative SenNP positive CD8 positive T cells, with axes labeled for CD3e, CD8a, SenNP, and CD44. Panel B shows similar flow cytometry plots for 5 microliters Ad SenNP immunized mice. Panel C presents vertical bar graphs quantifying total IV positive CD8 positive T cells in the nasal cavity, BAL, and lung for both 30 microliters and 5 microliters Ad SenNP immunized mice, with axes labeled for the number of cells and cavity type. Panel D shows vertical bar graphs quantifying IV positive SenNP positive CD8 positive T cells in the same cavities and immunization conditions. The graphs include statistical significance markers indicating differences between groups.
IV+ CD8 + T cells in the respiratory tract of Ad-SenNP 30 μl and 5 μl i.n. immunized mice. (A and B) Representative flow cytometry plots showing the frequency of IV+ or IV− SenNP+ CD8+ T cells in the nasal cavity, BAL, and lung of Ad-SenNP 30 μl (A) and Ad-SenNP 5 μl (B) immunized mice. (C and D) Quantification of total IV+ CD8 T cells (C) or IV+ SenNP+ CD8 T cells (D) in the nasal cavity, BAL, and lung of Ad-SenNP 30 μl (n = 10) and Ad-SenNP 5 μl (n = 10) immunized mice. Statistical significance was determined using a two-sided Mann–Whitney test with **P < 0.01, ***P < 0.001, and NS for not significant.