Panel A: A UMAP plot showing blood cells colored by cell type, including N/A, B cells, T cells, Monocytes, Neutrophils, and NK cells/Cytotoxic T cells. Panel B: A UMAP plot showing HYOU1 normalized counts for Patient 1 with red dots indicating neutrophil clusters. Panel C: A UMAP plot showing HYOU1 normalized counts for controls with red dots indicating neutrophil clusters. Panel D: A UMAP plot showing blood cells for Patient 1 and controls, with blue representing Patient 1 and red representing controls. Panel E: A UMAP plot showing reclustering of blood neutrophils, with blue representing patient cells and red representing control cells, labeled as NC1 and NC2. Panel F: A volcano plot showing differentially expressed genes between neutrophil cluster 1 and cluster 2. Panel G: A volcano plot showing differentially expressed genes between patients' neutrophils and controls' neutrophils. Panel H: Gene Set Enrichment Analysis plots displaying the enrichment of significantly upregulated pathways IFN α/β signaling and IFN γ signaling pathways in NC1 compared to NC2.
scRNA-seq analyses of blood cells. (A) UMAP plot showing blood cells colored by cell type. (B and C) UMAP plot showing HYOU1 normalized counts for the patient (B) and the controls (C) in blood as red dots (NC, neutrophil clusters). (D) UMAP plot showing blood cells for the patient and controls; colors correspond to group. (E) Reclustering of blood neutrophils shown in UMAP plot (blue, patient cells; red, control cells). (F and G) Volcano plots showing the differentially expressed genes between neutrophil cluster 1 and cluster 2, and between patients’ neutrophils and controls’ neutrophils, respectively. (H) GS EA plots displaying the enrichment of significantly upregulated pathways IFNα/β signalling and IFNγ signalling pathways in NC1 compared with NC2. FDR, false discovery rate; NES, normalized enrichment score. Analysis was performed in WebGestalt using weighted Kolmogorov–Smirnov statistic, with obtained P values from permutation testing and adjusted using FDR (Benjamini–Hochberg).