Panel A shows a bar graph comparing Gene Set Enrichment Analysis (GSEA) scores between parental and Trp53 minus/minus HPC7 cells. The x-axis lists different gene sets, and the y-axis represents the Normalized Enrichment Score (NES). Panel B displays Western blot results showing protein bands for different conditions. The lanes are labeled with conditions such as BioID-HA, Nuclear Localization Signal (NLS), and Promyelocytic Leukemia Protein fused with Retinoic Acid Receptor Alpha (PML fused with RARA), with or without biotin and Retinoic Acid (RA) treatment. Panel C is a scatter plot illustrating the fold change and statistical significance of PML fused with RARA interactors in HPC7 cells. The x-axis represents fold change (log base 2), and the y-axis represents the negative log base 10 p-value. Red dots indicate overlap with arsenic trioxide (ATO)-induced Small Ubiquitin-like Modifier (SUMO)-modified proteins. Panel D features a Venn diagram comparing PML fused with RARA interactors identified in HPC7 BioID and Lin minus progenitor TurboID datasets. The diagram shows overlapping and unique proteins identified in each dataset. Below the Venn diagram, tables list the unique and shared proteins categorized as transcriptional regulators, Mitogen-Activated Protein Kinase (MAPK) signaling, SUMO/ubiquitin, DNA repair and integrity, and other functional groups. Proteins known to be direct or indirect regulators of PML and RARA are highlighted in blue and red, and RARA fusion partners are underlined.
PML::RARA-interacting proteins. (A) GSEA comparing parental and Trp53−/− HPC7 cells; P value was indicated on the bottom of each bar. (B) Western blot analysis of lysate- and streptavidin-purified fractions from HPC cell lines expressing NLS-BioID or PML::RARA-BioID with or without 24-h ATRA treatment. N = 2. (C) Fold change and statistical significance of PML::RARA interactors in HPC7 cells (this study). Overlap with ATO (1 h)-induced sumoylated proteins previously identified in primary mouse APL blasts is indicated by red dots. (D) Comparison of PML::RARA interactants identified in HPC7 BioID and Lin− progenitor TurboID (Katerndahl et al., 2024). The protein list here was generated by the cutoff at log2 fold change >2.5 and FDR <0.05 in Lin− progenitor TurboID or FDR <0.01 in HPC7 BioID. The proteins labeled in blue and red are known direct/indirect regulators of PML and RARA. RARA fusion partners are underlined. Source data are available for this figure: SourceData FS1.