Figure 7.
Panel A shows two line graphs representing outside-out patch clamp recordings of rat AMPARs in HEK cells. The left graph displays desensitizing currents in response to a 500 millisecond application of glutamate, with the y-axis labeled in picoamperes and the x-axis in milliseconds. The right graph shows deactivating currents in response to a 1 millisecond pulse of glutamate, with similar axis labels. Panel B presents two line graphs for Hofstenia miamia AMPARs, with the same structure and labels as Panel A. Panel C consists of three bar graphs summarizing monoexponential fits to currents, with the y-axes labeled in milliseconds and the x-axes indicating different AMPAR types. The bar graphs compare time constants for activation, desensitization, and deactivation, with mean and standard deviation values indicated.
Fast activation and deactivation of diverse AMPARs. (A and B) Outside-out patch-clamp recordings of rat and H. miamia AMPARs in HEK cells. Left, desensitizing currents in response to 500 ms application of glutamate (10 mM). Right, deactivating currents in response to a 1 ms pulse of glutamate (10 mM). Holding potential was −60 mV. (C) Summary of monoexponential fits to currents, such as the ones shown in A and B. Time constants for activation, desensitization, and deactivation (τact, τdesens, and τdeact) are mean ± SD. P value from two-tailed unpaired t test.