The tree shows the evolutionary relationships among various species, with branches indicating genetic divergence. Key labeled nodes include Human-EMP1, Human-PMP22, Rat-GSG1, Rat-CACNG1, and Rat-CACNG6, among others. The tree is divided into two main groups: CACNG and TARP, each containing multiple sub-branches. The diagram includes percentage values at branch points, representing bootstrap support values. The tree is based on a manually trimmed alignment of 65 TARP-like sequences, with human EMP1 and PMP22 used as outgroups.
TARP-CACNG-GSG1 gene tree (manually trimmed alignment). Maximum likelihood tree based on manually trimmed alignment of 65 TARP-like sequences (see Materials and methods, Molecular phylogenetics). Alignment and tree downloadable as Data S5 and S6. Inset table shows full species names; superscript numbers indicate databases searched (websites listed in Materials and methods): 1, KEGG; 2, OIST; 3, published Xenacoelomorph datasets (Andrikou et al., 2019); 4, NCBI/GenBank; 5, UniProt; 6, LanceletDB; 7, Saccoglossus genome v3; 8, published Schizocardium datasets (Lin et al., 2024); 9, ENSEMBL Metazoa. Human EMP1 and PMP22 from UniProt. There are more genes in the overarching family outside of these branches e.g., EMP1 and PMP22, but we excluded most of them and used only human EMP1 and PMP22 as outgroups, based on previous work on this family (Ramos-Vicente and Bayes, 2020).