Panel A shows example recordings and a bar graph. The bar graph has current amplitude on the y-axis and different conditions on the x-axis. Panel B presents an example recording and a line graph of mean normalized current amplitude in response to increasing concentrations of glutamate at Rat-GluA2. The line graph has normalized current amplitude on the y-axis and glutamate concentration on the x-axis. Panel C includes example recordings, a bar graph of mean and individual oocyte current responses to glutamate, and a line graph of mean current in response to increasing glutamate concentrations for indicated cRNA combinations. The bar graph has current amplitude on the y-axis and different cRNA combinations on the x-axis, while the line graph has normalized current on the y-axis and glutamate concentration on the x-axis. Panel D shows example recordings of the effects of cyclothiazide on glutamate-gated currents and a bar graph of mean and individual data for CTZ-induced changes in glutamate-gated current. The bar graph has percentage change on the y-axis and different conditions on the x-axis. Panel E displays example recordings of glutamate-gated currents through indicated rat AMPARs. Panel F shows example recordings of indicated Hofstenia miamia AMPARs. Panel G presents example recordings, a bar graph of mean and individual glutamate-gated current amplitudes in oocytes expressing indicated WT and mutant Q1R Rat-GluA2 with or without human TARP-2. The bar graph has current amplitude on the y-axis and different conditions on the x-axis.
Functional characterization of diverse AMPARs. (A) Example recordings and mean (columns) current amplitude of responses to glutamate (1 mM) in oocytes injected with S. kowalevskii GluA RNA alone; alone and then with concanavalin A (ConA) treatment; and when co-injected with Saccoglossus TARP-XP_006822518.1. (B) Example recording of glutamate selectivity of Rat-GluA2 and mean ± SD (n = 4) normalized current amplitude in response to increasing concentrations of glutamate at Rat-GluA2. (C) Left, example recordings; and middle, mean (column) and individual oocyte current responses to 1 mM glutamate. Black data points from one batch of oocytes/experiments; cyan from another. Data were compared within batches, with one-way ANOVA and Tukey’s multiple comparisons test; selected P values shown; all other columns not significantly different from water-injected oocytes from that batch. Right, mean ± SD (n = 5) current (normalized to maximum) in response to increasing glutamate concentrations for indicated cRNA combinations. P value for unpaired t test of EC50s. (D) Left/middle, example recordings showing the effects of cyclothiazide (CTZ, 100 µM) on glutamate (1 mM) -gated currents. Right, mean (column) and individual data (dots) for CTZ-induced percent increase in glutamate-gated current or percentage of inhibition of glutamate-gated current. (E) Example recordings of glutamate-gated currents through indicated rat AMPARs, all scale bars as indicated top-left. (F) Example recordings of indicated H. miamia AMPARs, all scaled according to bars at right. (G) Example, mean (column), and individual glutamate-gated current amplitudes (−80 mV) in oocytes expressing indicated WT and mutant Q1R Rat-GluA2 with or without human TARP-γ2.