Figure S4.
The amino acid sequence alignment of AMPAR subunits from various species, including Rat, Sac, Lin, and Hof. Each sequence is annotated with predicted signal peptides and structural domains. The sequences are labeled with their respective species and subunit types. The alignment highlights conserved regions and differences among the sequences. Specific residues are marked with symbols such as hashtag and asterisk, indicating mutations and important residues discussed in other figures. The structural domains are inferred from 3D GluA2 structures and include extracellular N-terminal domains, ligand-binding domains, and transmembrane regions. The alignment is based on data from multiple sources, including SignalP 6.0 predictions and PDB structures.
Amino acid sequence alignment of AMPAR subunits studied here. The seven subunits were aligned with MAFFT (Katoh et al., 2002). Except for rat GluA2, indicated signal peptides are predictions from SignalP 6.0 (Teufel et al., 2022), with SignalP 6.0 likelihoods (where 1 is maximum) of 0.9991 for Sac-GluA, 0.0633 for Lin-g8707, 0.9992 for Lin-g16613, 0.9048 for Hof-98007595, 0.0663 for Hof-98019615, and 0.0432 for Hof-98014323. Indicated structural/functional domains are inferred from 3D GluA2 structures PDB accession no. 5WEO (Twomey et al., 2017) and PDB accession no. 1FTJ (Armstrong and Gouaux, 2000). #, amino acid residues mutated in Fig. 3 B. *, Q1 and Q2 (or R2) residues discussed in Fig. 4 and Fig. 5 E.