Figure 2.
Panel A shows a phylogenetic tree illustrating the lineage-specific complements of AMPAR genes across different species, including Rattus norvegicus, Saccoglossus kowalevskii, Lingula anatina, and Hofstenia miamia. Panel B presents current traces and a line graph showing mean current amplitude in response to increasing concentrations of glutamate (Glu) at oocytes injected with Sac-GluA and Sac-TARP-XP2518 cRNAs. The line graph has a logarithmic x-axis labeled [Glu] (M) and a y-axis labeled I/Imax. Panel C displays current traces and line graphs for Lingula anatina AMPAR genes, with similar axes as Panel B. Panel D shows current traces and line graphs for Hofstenia miamia AMPAR genes, again with similar axes. Each panel includes example current traces recorded from oocytes in response to amino acid applications and mean current amplitude graphs with error bars representing standard deviation.
Obligate heteromeric function of AMPAR genes that duplicated independently. (A) Lineage-specific complements of AMPAR genes. (B–D) Current traces, example currents recorded from oocytes injected with single AMPAR cRNAs or combinations of different AMPAR cRNAs, as indicated, in response to ∼10 s application of 1 mM Glu, or other amino acids. Graphs, mean ± SD (n = 4–5) current amplitude (normalized to maximum current amplitude) in response to increasing concentrations of Glu at oocytes injected with indicated cRNAs. Sac-TARP-XP…2518 in B refers to XP_006822518.1.