Figure S1.
The tree is based on a manually trimmed alignment of 386 sequences, with plant GLRs used as an outgroup. Branch support values are indicated by SH-aLRT and ultrafast bootstrap. The tree is color-coded to differentiate between various groups such as Plant GLRs, Cnidarian plus Bilaterian NMDAR genes, Cnidarian plus Bilaterian Epsilon genes, Cnidarian AKDF genes, Xenoturbella AKDF genes, and Bilaterian Delta genes. Specific branches are labeled with support values, and species names are listed along the branches. The inset table provides full species names and indicates the databases used for each sequence. The diagram includes labels for Kainate and AMPA receptors, highlighting their positions within the tree.
Animal iGluR gene tree (manually trimmed alignment). Maximum likelihood tree based on manually trimmed alignment of 386 cnidarian and bilaterian iGluRs and two plant GLRs as outgroup (see Materials and methods, Molecular phylogenetics). Branch support values are SH-aLRT and ultrafast bootstrap. Full alignment and tree available in Data S1 and S2. Inset table shows full species names; superscript numbers indicate databases used (websites listed in Materials and methods): 1, KEGG; 2, OIST; 3, published Xenacoelomorph datasets (Andrikou et al., 2019); 4, NCBI/GenBank; 5, UniProt; 6, LanceletDB; 7, Saccoglossus genome v3; 8, published Schizocardium datasets (Lin et al., 2024); 9, ENSEMBL Metazoa.